Coding

Part:BBa_I716211:Experience

Designed by: Samantha Liang   Group: iGEM07_Berkeley_UC   (2007-06-05)
Revision as of 18:07, 27 October 2010 by Pmboyle (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I716211

10/27/10: This part induces cell death when expressed. We (the 2010 Harvard iGEM team) received this part from the Anderson lab at Berkeley as part of an arabinose-inducible Barnase device that also contained a constitutive Barstar. Barstar is another protein that prevents Barnase from killing the cell. In our experience Barstar is required to even be able to clone Barnase, because Barnase is so potent.

User Reviews

UNIQ87a02702539ae537-partinfo-00000000-QINU

BBa_I716211 1 Not understood Pmboyle

10/27/10: Part works well, arabinose inducible Barnase caused cell death in a dose dependent manner. Please see http://2010.igem.org/Team:Harvard/fences/results for more info.

;

UNIQ87a02702539ae537-partinfo-00000002-QINU