Part:BBa_K320004:Experience

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Applications of BBa_K320004

This BioBrick has two main fonction, it makes the bacteria to resistant to the Antibiotic Kanamycin and it provides an origin of replication working for E.Coli and Asaia.

To test the BioBrick, we added it in the pSBC3 vector. This plasmid provides a resistance to Chloramphenicole and an origin of replication which works only for E.Coli.

We transfected both E.Coli and Asaia with the plasmid.

The transfected E.Coli were grown on a plate of LB with Agar containing Kanamycin with a concentration of . This experiment allows us to confirm that our part provides a resistance to Kanamycin.

The transfected Asaia were grown on a plate of Gly with Agar containing Kanamycin with a concentration of ...(according to our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia "characterisation of Asaia"]). This experiment allows us to confirm that the origin of replication and that Asaia aquired resistance to Kanamycin.

For each strand we made a negativ control with E.Coli or Asaia without plamsid.

We observe that on both plates with transfected bacteria we obtain colonnies and that in negativ controls we obtain non colonnies.

Put Picture of the plates

In conclusion we assume that our resitance to Kanamycin works and that the origin in replication also works for Asaia.

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