Coding

Part:BBa_I15010:Design

Designed by: Jeff Tabor   Group: iGEM04_UTAustin   (2004-09-20)
Revision as of 16:38, 14 July 2006 by Macowell (Talk | contribs) (→‎References)


cph8 (Cph1/EnvZ fusion)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2257
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutation at base 108 (G-A) to remove PstI site


Source

Voigt Lab via Anselm Levskaya

References

  • [Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71 www.pnas.org/cgi/doi/10.1073/pnas.191375198].