Composite

Part:BBa_K395402:Experience

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 13:11, 27 October 2010 by ODDY (Talk | contribs) (→‎Applications of BBa_K395402)

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Applications of BBa_K395402

In order to measure LacIM1(BBa_K395400), we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT. Furthermore, we confirmed that lacIWT shows stronger repression than LacIM1.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.



Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera

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