Regulatory
Strong

Part:BBa_K320002:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 12:33, 27 October 2010 by Spaceman (Talk | contribs) (→‎Source)

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Strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.


References

  1. [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]