DNA
Ori

Part:BBa_K320000:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-17)
Revision as of 12:24, 27 October 2010 by Spaceman (Talk | contribs) (→‎Source)

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Asaia replication origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal AgeI site found at 1320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was obtained through PCR.

Source

This BioBrick was obtained from pHM2-GFP [http://www.pnas.org/content/104/21/9047.long [1]]a strand of Asaia containing a plasmid with a GFP-gene. This plasmid was created by the team of Prof. Guido Favia.

References

  1. [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector]
  2. [http://aem.asm.org/cgi/content/abstract/68/5/2619 Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens]