Regulatory

Part:BBa_K427004

Designed by: Jan Marte Contreras Ortiz   Group: iGEM10_Tec-Monterrey   (2010-10-22)
Revision as of 11:15, 27 October 2010 by Janmarte (Talk | contribs)

Pmom promoter of Mu bacteriophage

Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.

Usage and Biology

The activation of the promoter requires the holenzyme σ70, which normally binds to the -35 and -10 consensus sequences, the promoter has the -10 but does not have the -35 sequences instead it has another sequence approximately at -51 where the activator protein binds. Once the protein is located there, the holenzyme σ70 recognizes the promoter and transcription begins.

This part has no special safety considerations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
positive_regulatorsBBa_K427001