Measurement

Part:BBa_K313035

Designed by: Masaya Fukushima, Ryosuke Kamei, Tomoka Ao, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-24)
Revision as of 11:02, 27 October 2010 by Pokeo (Talk | contribs)

Hin invertase assay device

This is a device that aims to test the recombination ability of the Hin DNA invertase.

Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will look red only when the cells express Hin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None