Regulatory

Part:BBa_K328003

Designed by: MEXICO UNAM CINVESTAV IRA   Group: iGEM10_Mexico-UNAM-CINVESTAV   (2010-10-26)
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cspA Cold box + UTR + SD +DB

This is Cold Box + UTR + SD + DB to test it with other promoters and measure them. More info in BBa_K328001

Warning!. The use of an Dowstream Box sequence in a coding device could lead the cell to a state of a "low temperature antibiotic effect (LACE)" by truncated RNA with the DB sequence. For more info see:

Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J. Biol. Chem. 272, 196–202.

This part was improved upon as https://parts.igem.org/Part:BBa_K2282011 where it was used for conditional expression of an amilCP reporter only at low temperature (~15 C).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None