Part:BBa_K317039
Pconst.(H)-RBS-LuxR-Double term-Plux-Lysis
This device senses the AHL concentration in the media and regulates the expression of downstream genes. The complex of the LuxR protein and the AHL activates the promoter, increasing the rate of transcription. Also, This devicce includes BBa_K112808 at the downstream gene, the auto lysis device which causing lysis of E.coli
Evaluation of BBa_K317039(Fig.3(A))
We performed a experiment with this device. The experiment was performed from single colonies in a streaked LB agar plate.At first, we cultured the single colony having the above device in 100 mL LB for 6 hors at 37℃ with agitation.Then, the culture was dispensed into 3 mL scale and AHL(f.c. 50 nM) was added to each dispensed culture to induce the expression of Lysis genes. The obtained culture was incubated for 20 hours at room temperature without agitation. After that, the OD660 of each culture(N=3) was measured.The result was shown below.
The result showed that the OD660 were different in presence of AHL(red bar) or not (gray bar).
OD660 of samples with the AHL were half less than no additive samples. That indicates that the complex of AHL and LuxR activated the expression of lysis genes and the E.coli in the culture were lysed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2400
Illegal NheI site found at 2423 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2005
Illegal AgeI site found at 2075 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal SapI site found at 2656
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