Regulatory
PompC-CS1

Part:BBa_K395303

Designed by: Taichi Nakamura   Group: iGEM10_Tokyo_Tech   (2010-10-02)
Revision as of 08:57, 27 October 2010 by Vancouver (Talk | contribs)

PompC(CS1) Promoter regulated by phosphorylated OmpR

This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(CS1) promoter consists of only binding site 1 before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. If you add E0240 as a reporter, this result is gained. Induction of new OmpC series in high osmolarity medium.
This work is done by Taichi Nakamura and Thiprampai THAMAMONGOOD
Tokyo Tech iGEM2010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None