RBS

Part:BBa_B0031:Experience

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.   Group: Antiquity   (2003-01-31)
Revision as of 16:12, 23 September 2011 by Nickpv (Talk | contribs) (User Reviews)

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Please enter how you used this part and how it worked out.

Applications of BBa_B0031

User Reviews

UNIQc1bdcf9688c49dae-partinfo-00000000-QINU

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQc1bdcf9688c49dae-partinfo-00000002-QINU


UNIQc1bdcf9688c49dae-partinfo-00000003-QINU

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UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

See Experience page of BBa_B0034for more details.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:

RBS effpLux effpTet effJ23101 Declared efficiency
B00310.401.68142.450,6
B00310.01ND0.040,07
B00320.190.41930.400,3
B00341111


Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI:

RBS effmRFP effAiiA effLuxI Declared efficiency
B00311.720.960.450,6
B00310.030.780.0280,07
B00320.370.51N.D.0.3
B00341111

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