Coding
crtE-AB

Part:BBa_K349101

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 03:44, 27 October 2010 by AlinaP (Talk | contribs)

crtE-AB (BioBytes 2.0)

One of the genes required in the lycopene production pathway for colour. Based of Cambridge 2009 E.Chromi project. Note that this is a "naked" ORF. It lacks a promoter, an RBS and a start and stop codon. It is engineered to have reconstituted promoter and terminator with adjoining BA Bytes using the BioBytes 2.0 assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 917


[edit]
Categories
Parameters
n/acrtE-AB (BioBytes 2.0)