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Coding
KanR-BA-Bs

Part:BBa_K349008

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)
Revision as of 02:55, 27 October 2010 by AlinaP (Talk | contribs)

KanR-BA-BsaI (for construction of BA BioBytes 2.0)

BioBytes 2.0 assembly method base plasmid v.1 containing the kanamycin resistance cassette. Digestion with BsaI allows for the addition of any other BA linker style part to the plasmid.


Usage and Biology

This plasmid is used to create BA linker style parts for the BioBytes 2.0 assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


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Categories
Parameters
n/aKanR-BA-BsaI (for construction of BA BioBytes 2.0)