Coding
RFP-BA-Bfu

Part:BBa_K349006

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 02:45, 27 October 2010 by AlinaP (Talk | contribs)

RFP-BA-BfuAI (for construction of BA BioBytes 2.0)

Base plasmid v.2 for the construction of new BioBytes 2.0. The RFP cassette can be removed using the BfuAI enzyme and a new BA linker type part may be inserted into the plasmid allowing for creation of parts for BioBytes Assembly Method 2.0.


Usage and Biology

The Base Plasmids v.2 are used to create parts for use in BioBytes 2.0 assembly method. The RFP coding device gives a selection method for finding plasmids with the part of interest in it.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRFP-BA-BfuAI (for construction of BA BioBytes 2.0)