Coding

Part:BBa_K399000:Design

Designed by: Alyssa Henning and Malinka Walaliyadde   Group: iGEM10_Cornell   (2010-10-26)
Revision as of 13:12, 27 October 2010 by Henninal (Talk | contribs) (→‎Design Notes)

mHoneydew


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mHoneydew contains a PstI site, so do not digest it with PstI. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and Xba1. Digest the upstream sequence at EcoRI and Spe1.

Source

The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.

References