Composite

Part:BBa_K374008:Experience

Designed by: Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Slodkowicz   Group: iGEM10_DTU-Denmark   (2010-10-20)
Revision as of 19:06, 26 October 2010 by MayaFriis (Talk | contribs) (→‎Biolector experiment)

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Applications of BBa_K374008

Biolector experiment

The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it pR promoter. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. From this construct no GFP expression is expected as repression of the pR promoter by the GogR repressor is tight. As a reference the repressor was removed from the construct allowing GFP expression from the pR promoter.


User Reviews

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