Part:BBa_K374008:Experience
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Applications of BBa_K374008
Biolector experiment
The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it pR promoter. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. From this construct no GFP expression is expected as repression of the pR promoter by the GogR repressor is tight. As a reference the repressor was removed from the construct and this was included in the biolector measurements.
User Reviews
UNIQ5c9b8e639b7d0748-partinfo-00000000-QINU UNIQ5c9b8e639b7d0748-partinfo-00000001-QINU