Coding
Bt-ADH

Part:BBa_K398005:Design

Designed by: Mathias Voges and Hugo F. Cueto Rojas   Group: iGEM10_TU_Delft   (2010-06-13)
Revision as of 14:50, 26 October 2010 by Hugo 87 (Talk | contribs) (→‎References)

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Medium-Chain Alcohol Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide sequence optimized for expression in E.coli. The restriction sites for XbaI, SpeI, EcoRI and PstI were removed. You can find the original sequence [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 here]

Source

Genbank: [http://www.ncbi.nlm.nih.gov/nuccore/AB040809.1 AB040809.1]

References

Kato,T., et al. Gene Cloning of an alcohol dehydrogenase from thermophilic alkane-degrading Bacillus thermoleovorans B23, J. Biosci. Bioeng. 91 (1), 100-102 (2001).