Part:BBa_K422015

pSEVA132

Victor de Lorenzo's lab, Ampicillin resistance, pBBR1 ori

Plasmid features

• Ampicillin resistance
• pBBR1 origin of replication

Design features

Compatible with the cloning strategy BBF RFC28.

The genes for the repressor protein AraC and the corresponding ParaBAD promotor/operator sites were introduced into the vector pSEVA 132 followed by an insert, which is flanked by AarI-recognition sites. Digest with AarI releases this insert and generates a vector with assembly-compatible overhangs.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 292
  Illegal EcoRI site found at 1505
  Illegal XbaI site found at 3478
  Illegal SpeI site found at 1
  Illegal SpeI site found at 3127
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 292
  Illegal EcoRI site found at 1505
  Illegal SpeI site found at 1
  Illegal SpeI site found at 3127
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 292
  Illegal EcoRI site found at 1505
  Illegal BglII site found at 1899
  Illegal BamHI site found at 1464
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 292
  Illegal EcoRI site found at 1505
  Illegal XbaI site found at 3478
  Illegal SpeI site found at 1
  Illegal SpeI site found at 3127
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 292
  Illegal EcoRI site found at 1505
  Illegal XbaI site found at 3478
  Illegal SpeI site found at 1
  Illegal SpeI site found at 3127
  Illegal NgoMIV site found at 5397
  Illegal NgoMIV site found at 5891
  Illegal NgoMIV site found at 6020
  Illegal AgeI site found at 1299
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1597
  Illegal SapI site found at 1281
  Illegal SapI site found at 3733
  Illegal SapI.rc site found at 3061
  Illegal SapI.rc site found at 3409