RNA

Part:BBa_K337016

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-21)
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synthetic microRNA HD10

Synthetic microRNA HD10 is a adaption of microRNA hsa-mir122 to a synthetic target sequence. microRNA HD10 is oriented reverse-complementary and can be driven with any Pol2 promoter of the users choice. This part can be used for the synthesis of any synthetic microRNA by applying the Team Heidelberg 2010 synthetic microRNA engineering protocol (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None