Part:BBa_K346021

PmerT promoter mutant 3+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)

We constructed this part to calculate the expression level of GFP downstream of the pmerT mutant 3. The response curve was characterized by using different concentration of Hg(II) to induce GFP's expression and measured the expression level. In detail, when the concentration of Hg(II) in the cytosol changes, the active form of merR dimer will increase and the possibility that it will active pmerT promoter will increase.

Figure 1: The GFP intensity when cultured by different concentration of Hg(II). Cultures were diluted 1:100 in LB and grown to final OD600 0.6. Then different concentration of Hg(II) was added to the culture and we put the tube in the shaker for 2 hours in 30 ℃. Then we suspended the culture with 1% PBS and measure the GFP intensity using a microplate reader.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 730