Regulatory

Part:BBa_K328001:Design

Designed by: MEXICO UNAM CINVESTAV IRA   Group: iGEM10_Mexico-UNAM-CINVESTAV   (2010-10-26)
Revision as of 06:28, 26 October 2010 by Cjdg (Talk | contribs) (→‎References)

cspA Promoter + Cold Box + UTR + SD + DB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was taken with pcr amplification from dh5a and is on tests


Source

PCR E coli dh5a

References

This is the complete promoter cspA promoter + Cold Box + UTR + SD + DB extracted from E coli dh5a for test about the cold shock response and the ability to control a genetic circuit using it as a cold sensor, is intended to produce proteins after a cold shift and allows us to sintetize thermo-labile proteins also.