Coding
crtB-AB

Part:BBa_K349102

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 01:31, 27 October 2010 by Phacking (Talk | contribs)

crtB-AB (BioBytes 2.0)

One of the protein required in the lycopene pathway. This is a naked ORF in that it does not include a promoter or RBS sequence. This part is based of the Cambridge 2009 E.Chromi project. The Promoter, and terminator are intended to be reconstituted with adjoining linker style Bytes in the BioBytes 2.0 Assembly Method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 700
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 936


[edit]
Categories
Parameters
n/acrtB-AB (BioBytes 2.0)