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This part contains BBa_K316007 with added double terminator BBa_B0014. This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. Terminator BBa_B0014 has been added to comply with Biobrick standards. This particular terminator is stronger and is different from BBa_B0015.

Structure and Features

Figure I. Graphical representation of the GFP-XylE construct with associated Pveg promoter, tags, linkers and double terminator.

Sequence and Features Status: 500 Content-type: text/html

Software error:

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Part Characterisation

Characterisation data was obtained using GFP-XylE constructs BBa_K316008 and XylE under two different promoters: B. subtilis derived Pveg BBa_K316005 and J23101 BBa_K316004 from E. coli. These are described on our wiki[http://2010.igem.org/Team:Imperial_College_London/Results] and the relevant parts pages.