Device

Part:BBa_K302035:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 01:14, 26 October 2010 by Swoodhouse (Talk | contribs) (→‎Design Notes)

Sucrose-limitation induced kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below.
  • Organisms with this device integrated must be grown on 10% sucrose media to be viable.
  • Should be integrated at the sacB locus to prevent native Levansucrose glue production.

Source

Bacillus Subtilis 168 strain

References

Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.

Shimotsu, H., and Henner, D. J. (1986) J. Bacteriol. 168, 380–388