Reporter

Part:BBa_K274003:Experience

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-18)
Revision as of 15:27, 27 October 2010 by DNesbeth (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274003

User Reviews

E. coli turned dark green after transformation of this plasmid. This is unexpected as there is no promoter upstream of the operon.

BBa_K274003 Review No.2 UCL_London, Xiang Chen

Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below.

This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.


K274003 Diagnostic Gel .jpg

UNIQd7e1f1cdc291a3fd-partinfo-00000000-QINU UNIQd7e1f1cdc291a3fd-partinfo-00000001-QINU