Protein_Domain

Part:BBa_K321003:Design

Designed by: Crystal Liu   Group: iGEM10_UCSF   (2010-10-25)
Revision as of 22:34, 25 October 2010 by Chiaywu (Talk | contribs) (→‎Design Notes)

intracellular chain of CD3 zeta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 274


Design Notes

A pre-existing PstI site was removed to meet the BioBrick standard. A PCR product encoding the sequence was flanked with an XbaI site at the 5' and an SpeI site at the 3' for ligation with XbaI, SpeI double-digested pSB1C3 vector.

Source

Cloned from the human genome.

References