Device

Part:BBa_K302012:Experience

Designed by: Rachel May Boyd, Phil Hall   Group: iGEM10_Newcastle   (2010-08-12)
Revision as of 21:32, 25 October 2010 by RachelBoyd (Talk | contribs) (→‎Applications of BBa_K302012)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K302012

Graph1:
Teamnewcastle yneA168.png
Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168.
Graph2:
Newcastle no induction.jpg
See below: Bacillus subtilis 168 cells (left) and non-induced cells(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle noindBS.jpg
Graph 2 shows the percentage of cells at different lengths(μm)uninduced
Graph3:
Newcastle 0.2 induction.jpgTeamnewcastle 0.2indBS.jpg
Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
Graph4:
Newcastle 1IPTG.jpgTeamnewcastle 1indBS.jpg
Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

User Reviews

UNIQ6cb8314bbb47270d-partinfo-00000000-QINU UNIQ6cb8314bbb47270d-partinfo-00000001-QINU