Device
Toggle
Part:BBa_K415300
Designed by: Leanna Morinishi, Grant Robinson, Andrew Yang, Paul Muir, Shirley Galbiati, Shawn Pan, Jason Stevens, Lauren McGough, Deepak Mishra, Ron Weiss Group: iGEM10_MIT (2010-10-23)
Revision as of 20:19, 25 October 2010 by Supacalafrglstic (Talk | contribs)
Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt
From Programmable cells: Interfacing natural and engineered gene networks, Kobayashi, H. et. al, PNAS.The [http://www.pnas.org/content/101/22/8414.full|Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
The following data was taken from cells co-transformed with pTSMa and Part:BBa_K415023.
UV Power Modulation | |||||
---|---|---|---|---|---|
Photos | |||||
FACS (in J/m^2) | |||||
0 | 5 | 20 | 40 |
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1773
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |