Generator
Ori+Kan

Part:BBa_K320004

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 19:26, 25 October 2010 by Perrudet (Talk | contribs)

Origin for Asaia and resistance to Kanamycin



 This BioBrick is constitued by an origin of replication for Asaia and E. coli (No part name specified with partinfo tag..) and a resistance to Kanamycin (No part name specified with partinfo tag..). It was initially designed to be inserted into (BBa_I51020.).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


[edit]
Categories
//cds/selectionmarker/antibioticresistance
//plasmid/component/origin
Parameters
None