Composite

Part:BBa_K364325:Experience

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)
Revision as of 23:05, 25 October 2010 by Lityupix (Talk | contribs) (→‎Applications of BBa_K364325)

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Physiological function of PXR

Like other type II nuclear receptors, when activated, it forms a heterodimer with the retinoid X receptor, and binds to hormone response elements on DNA which elicits expression of gene products.

One of the primary targets of PXR activation is the induction of CYP3A4, an important phase I oxidative enzyme that is responsible for the metabolism of many drugs. In addition, PXR up regulates the expression of phase II conjugating enzymes such as glutathione S-transferase and phase III transport uptake and efflux proteins such as OATP2 (SLCO2A1)and MDR1(ABCB1).

Possible applications of GAL4-PXR

By the addition of lipid ligand, the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364325

Picture of gel electrophoresis: Gal4-PXR in pCDNA3.1 resulting an insert of 1181 bp.
Picture of Western blot: Gal4-PXR composite construct is highly expressed after transferring it into pCDNA3.1

User Reviews

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