Reporter

Part:BBa_K362052:Design

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)
Revision as of 15:16, 26 October 2010 by Takeuchi (Talk | contribs) (→‎Design Notes)

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ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2911
    Illegal BamHI site found at 2900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
    Illegal AgeI site found at 1581
    Illegal AgeI site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3603


Design Notes

This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_I13507),RFP(BBa_I13507) and 2 terminators(BBa_I13507),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013),sufA promoter(BBa_K362005),RBS(BBa_K121013),GFP(BBa_K121013),2 terminators(BBa_K121013). sufA promoter has sensitibity to oxydative stress. ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription facor. sufA promoter has sensitivity to oxidative stress.

Source

sufA Promoter from E.coli genome

References