Reporter

Part:BBa_K362007:Design

Designed by: Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura   Group: iGEM10_KIT-Kyoto   (2010-10-13)
Revision as of 09:37, 25 October 2010 by Nakamura (Talk | contribs) (→‎Design Notes)

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This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1034
    Illegal BamHI site found at 1023
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1726


Design Notes

This composite part has the promoter ,ahpC-promoter controlled by OxyR protein,and GFP ,double terminator.

Source

This composite part has the promoter ,ahpC which has sensitivity to oxidative stress and GFP , terminator.


AhpC.jpg

This graph shows that the fluorescent intensity of the expression system in which ahpC-promoter controls the GFP gene.

References