Coding

Part:BBa_K323214:Experience

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-25)
Revision as of 17:30, 25 September 2012 by LucijaK (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K323214

User Reviews

[http://2012.igem.org/Team:Slovenia 2012 iGEM team Slovenia ] discovered that NicTAL part deposited in the Registry by the Slovenian iGEM2010 team was missing subdomain in DNA-binding domain, so our team added this missing part on N terminal sequence. Apart from adding N terminal sequence, we added HIS tag on N terminal end and NLS on terminal C end of NicTAL12 (Figure 1).

Svn 12 NicTAL10vs12.PNG

Figure 1:Sequence alignment of NicTAL10 and NicTAL12, showing differences between constructs.


Results obtained by testing NicTAL12:KRAB repressor proved that NicTAL12 binds to its binding sites better than NicTAL10.

Svn 12 NicTAL graf.png

Figure 2: Results of testing NicTAL12:KRAB repressor shows, that NicTAL12 binds to DNA better than NicTAL10.



UNIQf0a1a919e39fa6af-partinfo-00000000-QINU UNIQf0a1a919e39fa6af-partinfo-00000001-QINU