DNA

Part:BBa_K419015:Experience

Designed by: YANG TUNG CHIN   Group: iGEM10_TzuChiU_Formosa   (2010-10-24)
Revision as of 17:40, 6 November 2010 by Hewyaohua (Talk | contribs) (→‎Applications of BBa_K419015)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K419015

Verification of the Part BBa_K419015

The Lux R gene with ribosome binding site (RBS) DNA was amplified from the part BBa_C0062. The Lux R PCR products (756 bp) were digested with EcoR I and Pst I, then ligated with the EcoR I-Pst I treated pSB1C3. After E. coli transformation and plasmid preparation, the part BBa_K419015 for sensing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the Lux R DNA fragment. The sensing system composite was transformed into E. coli for further experiments.

Photo14.jpg

User Reviews

UNIQ653348dd1aac2165-partinfo-00000000-QINU UNIQ653348dd1aac2165-partinfo-00000001-QINU