Composite

Part:BBa_K419012:Experience

Designed by: YANG TUNG CHIN   Group: iGEM10_TzuChiU_Formosa   (2010-10-24)
Revision as of 13:19, 6 November 2010 by Hewyaohua (Talk | contribs) (→‎Applications of BBa_K419012)

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Applications of BBa_K419012

The Part BBa_k419012 (constitutive promoter-CrtEBIY)Verification of the Part BBa_k419012

We use constitutive promoter to drive the CrtEBIY composite for beta-carotene synthesis. The CrtEBIY composite (BBa_K274210) was obtained from the distribution part of iGEM 2009 Cambridge and digested with restriction enzymes, EcoR I and Pst I, then ligated to EcoR I and Pst I sites of pSB1C3, the iGEM 2010 standard backbone. We verified our composite for synthesis system by EcoRI and PstI digestion and the agarose gel electrophoresis result was shown. The arrow (red) indicates the CrtEBIY with constitutive promoter (R001) DNA fragment. The synthesis system composite was transformed into DH5-alpha E. coli for further experiment.

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