Measurement

Part:BBa_K313035

Designed by: Masaya Fukushima, Ryosuke Kamei, Tomoka Ao, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-24)
Revision as of 10:48, 27 October 2010 by Pokeo (Talk | contribs)

Hin invertase assay device

This is a device that aims to test the recombination ability of Hin DNA invertase. This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."

lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None