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Part:BBa_K415148:Experience

Designed by: Jason Stevens   Group: iGEM10_MIT   (2010-10-24)
Revision as of 06:06, 25 October 2010 by Galbiati (Talk | contribs) (→‎Applications of BBa_K415148)

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Applications of BBa_K415148

Assessing p8-Jun incorporation into phage coat

We transformed XL1-Blue cells with BBa_K415149, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.

There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.

User Reviews

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