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Part:BBa_K415147:Experience

Designed by: Jason Stevens   Group: iGEM10_MIT   (2010-10-24)
Revision as of 06:04, 25 October 2010 by Galbiati (Talk | contribs) (→‎Applications of BBa_K415147)

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Applications of BBa_K415147

Assessing p8-Fos incorporation into phage coat

We transformed XL1-Blue cells with BBa_K415147, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody.

1023westernfos.jpg

The purple band at 13 kD represents the p8-Fos fusion, and is evidence of the fusion protein's incorporation into the phage coat. We are currently looking into possible explanations for the fainter band at 15 kD.

User Reviews

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