Coding

Part:BBa_K364315

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-01)
Revision as of 13:43, 25 October 2010 by Mrtwister (Talk | contribs)

NHR-23 LBD

Nuclear hormone receptor family member 23. The nhr-23 gene encodes a nuclear hormone receptor homolog that is required in all larval molts. NHR-23 is highly similar to Drosophila DHR3, an ecdysone-inducible gene product involved in metamorphosis. The NHR-23 protein is nuclear, and is present in all blastomeres during early embryogenesis. During later stages of morphogenesis, NHR-23 is restricted to epidermal cells. The expression of nhr-23 cycles between stages of larval development: during each intermolt period, levels of nhr-23 transcripts are 2-5 times greater than levels at each molt. Furthermore NHR-23 binds the DRS-type hormone response sequence in vitro.

Picture of gel electrophoresis: NHR 23 in pSB1C3 results a 495 bp long insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 76
    Illegal BsaI.rc site found at 382
    Illegal BsaI.rc site found at 466


[edit]
Categories
Parameters
n/aNHR-23 LBD