Part:BBa_K364303:Experience

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Physiological effect

daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.

Applications of BBa_K364303

DAF-12 LBD can be fused with various DBDs to activate the expression of chosen genes by addition of dafachronic acids (ligand).

References

Antebi A, Yeh WH, Tait D, Hedgecock EM, Riddle DL. daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes Dev. 2000 Jun 15;14(12):1512-27.

Snow MI, Larsen PL.Structure and expression of daf-12: a nuclear hormone receptor with three isoforms that are involved in development and aging in Caenorhabditis elegans. Biochim Biophys Acta. 2000 Nov 15;1494(1-2):104-16.

Matyash V, Entchev EV, Mende F, Wilsch-Bräuninger M, Thiele C, et al. (2004) Sterol-Derived Hormone(s) Controls Entry into Diapause in Caenorhabditis elegans by Consecutive Activation of DAF-12 and DAF-16. PLoS Biol 2(10): e280.

Motola D.L., Cummins C.L., Rottiers V., Sharma K.K., Li T., Li Y., Suino-Powell K., (...), Mangelsdorf D.J. Identification of Ligands for DAF-12 that Govern Dauer Formation and Reproduction in C. elegans. (2006) Cell, 124 (6), pp. 1209-1223.

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