Composite

Part:BBa_K364328:Experience

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)
Revision as of 23:21, 25 October 2010 by Lityupix (Talk | contribs) (→‎Applications of BBa_K364328)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Physiological effects of DAF-12

daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.

Possible applications of GAL4-DAF-12

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364328

Picture of gel electrophoresis: Gal4-DAF12 in pCDNA3.1 resulting an insert of 971 bp.

User Reviews

UNIQ3b63e3fa31eef43b-partinfo-00000000-QINU UNIQ3b63e3fa31eef43b-partinfo-00000001-QINU