Composite

Part:BBa_K364328:Experience

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)
Revision as of 23:21, 25 October 2010 by Lityupix (Talk | contribs) (→‎Applications of BBa_K364328)

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Physiological effects of DAF-12

daf-12 in Caenorhabditis elegans plays a key role in the regulation of development and determination of adult longevity. It has effects also on resistance to oxidative and thermal stress which makes daf-12 the first gene with alleles that can extend or shorten lifespan.

Possible applications of GAL4-DAF-12

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364328

Picture of gel electrophoresis: Gal4-DAF12 in pCDNA3.1 resulting an insert of 971 bp.

User Reviews

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