Regulatory
Strong

Part:BBa_K320002:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 12:32, 27 October 2010 by Spaceman (Talk | contribs) (→‎Design Notes)

Strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.

Source

The source is the article [http://www.pnas.org/content/104/21/9047.long [1]].

References

  1. [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]