Device
Toggle

Part:BBa_K415300

Designed by: Leanna Morinishi, Grant Robinson, Andrew Yang, Paul Muir, Shirley Galbiati, Shawn Pan, Jason Stevens, Lauren McGough, Deepak Mishra, Ron Weiss   Group: iGEM10_MIT   (2010-10-23)
Revision as of 18:16, 23 October 2010 by Supacalafrglstic (Talk | contribs)

Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt

From Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.

The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

CollinsToggle.png
The







Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None