Coding

Part:BBa_K364202

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-08-10)
Revision as of 07:44, 24 October 2010 by Mrtwister (Talk | contribs)

hBAX

Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).

Picture of gel electrophoresis: hBAX in pSB1C3 resulting an insert of 573 bp. Circular pSB1C3-hBAX and restricted PMAT-hBAX can be seen as control on the gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None