Coding

Part:BBa_K364202

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-08-10)
Revision as of 13:40, 23 October 2010 by Mrtwister (Talk | contribs)

hBAX

Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis which is the most frequent type of PCD (Programmed cell death).

Picture of gel electrophoresis: hBAX in pSB1C3 resulting an insert of 573 bp. Circular pSB1C3-hBAX and restricted PMAT-hBAX can be seen as control on the gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None