Part:BBa_K346088

RBS(BBa_B0034)+MerC

This part is constructed for the expression of merC. MerC can transport Hg(II) into the cytosol without the help of merP. For a long time, merC is considered to be of no use in the resistance to Hg(II) because mutations that affect the expression of MerC did not affect the resistance. The discovery in Thipobacillus isolated from mercury mines of operons that apparently had only merC and no merT genes forced reconsideration of the view that merC is vestigal. It has been proposed that merC may be needed under conditions of very high Hg (II) exprosure, perhaps because merC system can uptake (Hg (II) without the need of merP.

We constructed this part to increase the amount of Hg(II) that can be uptaked into the cell. Since the more Hg(II) in the cell, the more merR dimer will bind Hg(II)and be activated. Those active merR dimer will cause the gene expression under pmerT. This part can thus increase the amount of expression of genes under pmerT by increase cellular Hg(II) concentration.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 384
  
• 1000
  COMPATIBLE WITH RFC[1000]