Part:BBa_K314200:Experience

Evidence that Tsi2 works as expected

This evidence can be found on [http://2010.igem.org/Team:Washington/Gram_Negative/Test this page] from the 2010 UW iGEM Wiki, under the heading "Evidence that Tse2 and Tsi2 are Function As Expected"

Applications of BBa_K314200

Tse2 is a potent bacterial toxin and thus has many applications, with or without its immunity protein, Tsi2. One potential application is use as a biotechnology agent in the way that the CcdB, the Death Protein, is used.

Tse2 could also be combined with the T6SS to result in a system that targets this toxin into the cytoplasm of target Gram-negative bacteria. This is useful to create Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing in response to a user-defined signal.

If Tse2 is combined with a functioning Type 6 Secretion System and the antitoxin Tsi2 it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application. This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].

Diagram of the Tse2/Tsi2/ Type VI Secretion System as it would be used in a probiotic application

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