Regulatory

Part:BBa_K337011

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-21)
Revision as of 14:54, 21 October 2010 by Kleinsorg (Talk | contribs)

hsa-miR-221 Binding site pattern (2BS)

hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None